看国外 | 海藻源β-葡聚糖可替代抗生素应对仔猪大肠杆菌感染

看国外 2019-03-11 13:30:17

来源:All About Feed

In the ideal situation when a pig is challenged with an E. coli infection, the infection is stopped by having a strong immune response, followed by a prompt reversion to normal growth. The use of algae-derived β-glucan can help do this.


β-glucans are a heterogeneous group of polysaccharides naturally present in cereal grains, fungi, seaweed, and algae and are known to have anti-tumor and immunomodulatory effects in vitro, although not all types of β-glucans exhibit similar immune-modulatory effects.


Euglena gracilis algae


Currently, the majority of commercially available β-glucans are derived from yeast, whereas limited research have been conducted on algae-derived β-glucan in pigs. The β-glucans extracted from algae Euglena gracilis are linked by (1,3)-glycosidic bonds and are categorised as paramylon.


Earlier research showed that β-glucan from algae Euglena gracilis strongly stimulated porcine leukocytes in vitro. Therefore, researchers wanted to know if supplementation of algae Euglena gracilis-derived β-glucan could modulate immune responses and gut integrity, and with this enhance disease resistance and health of weaned pigs. 


The objectives of this study were to investigate the effects of algae-derived β-glucan on diarrhoea, gut permeability and immune responses of weaned pigs experimentally infected with F18 E. coli. The study was published in the Journal of Animal Feed Science and Technology.



Photo: Henk Riswick

3 dietary treatments


A total of 36 weaning pigs (21 days old) with an equal number of gilts and barrows and an average initial body weight (BW) of 7.69 ± 0.77 kg were selected from the Swine Teaching and Research Center of the University of California, Davis. The sows and piglets used in this experiment did not receive E. coli vaccines, antibiotic injections, or antibiotics in creep feed. After weaning, all pigs were randomly assigned to one of 3 dietary treatments:

从加利福尼亚大学戴维斯分校的猪教学和研究中心选取36头断奶猪(21天日龄),后备母猪和阉猪的数量相等,平均初始体重(BW)为7.69±0.77 kg。本实验所用母猪和仔猪未接种大肠杆菌疫苗、抗生素注射剂或蠕变饲料中的抗生素。断奶后,随机给所有的猪分配以下3种喂食方案:

1) a complex nursery basal diet as the control diet


2) a complex nursery basal diet including 54 mg/kg (low dose) of algae-derived β-glucan

2)复合保育基础饮食,添加54 mg/kg(低剂量)藻类源β-葡聚糖

3) a complex nursery basal diet including 108 mg/kg (high dose) of algae-derived β-glucan

3)复合苗圃基础饮食,添加108 mg/kg(高剂量)藻类源β-葡聚糖

All diets met the current estimates for nutrient requirements for nursery pigs (NRC, 2012). Spray-dried plasma, antibiotics, and zinc oxide were not included in the diets.


The experimental diets were fed to pigs as a mash form throughout the experiment. The β-glucan used in this experiment was ProGlucan™ (dried algae Euglena gracilis) and provided by Algal Scientific, USA (Plymouth, MI), part of Kemin. The product contained around 50% β-glucan, in which 95% was β-1,3-glucan. After 5 days adaptation, all pigs were orally inoculated with 3 mL of F18 E. coli for 3 consecutive days from d 0 post-inoculation (PI).

在整个实验过程中,实验性饲料以糊状的形式喂给猪。本实验中使用的β-葡聚糖是ProgLucan™(干海藻Eugena gracilis),由美国Algal Scientific(普利茅斯,密歇根州)提供,该公司隶属于建明。该产品含有约50%的β-葡聚糖,其中95%为β-1,3-葡聚糖。经过5天适应期之后,连续3天(自接种当天算起)给所有猪口服接种3 ml F18大肠杆菌。

Acceleration of infection recovery


Results of this experiment revealed that inclusion of high dose β-glucan reduced frequency of diarrhoea (29.01% vs. 17.28%) for the entire experimental period. The feed supplementation of approximately 108 mg/kg of algae-derived β-glucan alleviated diarrhoea of F18 E. coli infected pigs by enhancing gut integrity. 

该试验结果表明,在整个试验期间,添加高剂量的β-葡聚糖可以降低腹泻发生率(29.01% vs 17.28)。由于增强了肠道完整性,日粮中补充添加108 mg/kg海藻源β-葡聚糖减轻了F18大肠杆菌感染猪的腹泻症状。

Feeding algae-derived β-glucan also boosted host immune response against E. coli infection. The β-glucan product stimulated T cell activation and reduced inflammation and, therefore, may accelerate the recovery of pigs from E. coli infection. The use of algae-derived β-glucan will promote weaned pig health and increase profitability of pork producers as the use of antibiotics in feed is restricted.







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